Protein-A ELISA kit

Protein-A is a type of bacterial surface protein that has a high affinity for binding to immunoglobulin (Ig) of mammalian species. In the purification process of monoclonal antibodies with protein A purification column, there is always the possibility of the presence of subtle amounts of this protein in the purified final product. The protein-A impurity test kit has the ability to detect small amounts of this protein in pharmaceutical products. Its work is based on the sandwich ELISA method, which is a technique based on Ag-Ab interaction and based on the binding power of the kit’s specific polyclonal antibody (coated in the bottom of the plate) and protein A contained in the monoclonal antibody, the intensity of the color will be different after binding the conjugated antibody with HRP for each of the samples, which indicates the amount of protein A in the product. After comparing the OD values obtained in the test for drug samples with standard samples with specific concentrations (available in the kit), the amount of protein A concentration in the product is obtained, and whether the product is allowed or not according to the LOD Market entry is determined.